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32 Results for "novus"

NB600-532

beta-Actin Antibody

(6 Reviews)

(63 Publications)

0.1 mg

NB600-532

Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin using NB600-532 at 1:200 in A431, C2C12 and C6 cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: beta-Actin Antibody [NB600-532] - Detection of actin in 3T3 lysates (20ug). ECL detection 30 seconds. A specific band is seen using different dilutions: Lane 1 (1:15,000), Lane 2 (1:10,000), and Lane 3 (1:15,000).

Western Blot: beta-Actin Antibody [NB600-532] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-532 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Western Blot: beta-Actin Antibody [NB600-532] - Mouse colon whole cell lysate. PVDF membrane was probed with Rabbit Anti-B actin Antibody (Catalog # NB600-532) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. WB image submitted by a verified customer review.

Immunohistochemistry: beta-Actin Antibody [NB600-532] - Detection of human Cytoskeletal Actin by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin (NB600-532). Detection: DAB

Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using RCC4 whole cell lysate [NBP1-30412].

Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].

Western Blot: beta-Actin Antibody [NB600-532] - Analysis using the HRP conjugate of NB600-532. Detection of Beta Actin in MCF-7 cell lysate (20ug) using anti-Beta Actin antibody. WB image submitted by a verified customer review.

Western Blot: beta-Actin Antibody [NB600-532] - Treatments of periplocin and/or TRAIL activate DR4, FADD, and proapoptotic proteins in HCC cells. The effect of periplocin treatment on the expression of DR4, DR5, and FADD was analyzed by western blot. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ecam/2013/958025/), licensed under a CC-BY license.

Western Blot: beta-Actin Antibody [NB600-532] - Hypoxic preconditioning increases PBMNC sensitivity to apelin-13 via upregulation of APJ expression, leading to growth factor secretion.(a) APJ protein expression was significantly increased in hypoxic PBMNCs. *p Mol Neurodegener (2020). Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, SyHa, Gp, Pm-Or, Rb, Sh, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, SyHa, Gp, Pm-Or, Rb, Sh, Xp

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-532AF350
Alexa Fluor 405	NB600-532AF405
Alexa Fluor 488	NB600-532AF488
Alexa Fluor 532	NB600-532AF532
Alexa Fluor 594	NB600-532AF594
Alexa Fluor 647	NB600-532AF647
Alexa Fluor 700	NB600-532AF700
Alexa Fluor 750	NB600-532AF750
Biotin	NB600-532B
DyLight 350	NB600-532UV
DyLight 405	NB600-532V
DyLight 488	NB600-532G
DyLight 550	NB600-532R
DyLight 594	NB600-532DL594
DyLight 650	NB600-532C
DyLight 680	NB600-532FR
DyLight 755	NB600-532IR
FITC	NB600-532F
HRP	NB600-532H
Janelia Fluor 549	NB600-532JF549
Janelia Fluor 646	NB600-532JF646

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NB200-192

DDX6 Antibody

(24 Publications)

100 ul

NB200-192

Western Blot: DDX6 Antibody [NB200-192] - Detection of Human and Mouse DDX6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody NB200-192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-192] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX6 Antibody [NB200-192] - Detection of human DDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-192 (lot NB200-192-3) used for IP at 6 ug per reaction. DDX6 was also immunoprecipitated by a previous lot of this antibody (NB200-192-2) and rabbit anti-DDX6 antibody NB200-191. For blotting immunoprecipitated DDX6, NB200-192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

<b>Genetic Strategies Validation. </b>Knockdown Validated: DDX6 Antibody [NB200-192] - Western blot analysis of HNRNPK and DDX6 protein levels upon HNRNPK or DDX6 knockdown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-12238-x), licensed under a CC-BY license.

Hypoxia and HIF-1 alpha /VEGF induction upon S. pneumoniae infection in brain ECs. a Control and S. pneumoniae infected bEnd5 cells treated with pimonidazole hydrochloride (Hypoxyprobe 200 mM) and visualized for cellular hypoxia by red 549-linked mouse anti-pimonidazole monoclonal antibodies. D39 and TIGR4 strains infected cells show significantly high number of hypoxic cells compared to control cells which is however not present in the top row without the probe indicating specificity of the staining (scale bar 10 µm). b Oxygen levels (%) were quantified in the medium of control and S. pneumoniae infected bEnd5 cells using the SensorDish® Reader (SDR) for non-invasive measurement of O2 levels through the transparent well bottom. O2 concentration (pO2% air saturation) was obtained at 60-s intervals and average values at several different time points shown indicate a significant reduction in O2 levels within minutes post-infection and to hypoxic levels by 2 h (N = 3, 2-tailed paired t test compared to control for each condition at the corresponding time point, mean ± SEM, **p < 0.01, ***p < 0.001), which was however not present either in control or deferoxamine (DFO, 200 µM), an iron chelator that induces HIF-1 alpha independent of hypoxia. c Control and S. pneumoniae infected bEnd5 cells were analyzed for HIF-1 alpha protein induction by Western blotting. The result shows a significant increase in HIF-1 alpha levels in both D39 and TIGR4 infected cells compared to control conditions when the band pixel intensity is quantified by normalizing to the housekeeping protein beta -actin band. DFO served as a positive control for HIF-1 alpha induction (N = 4, *p < 0.05, 2-tailed paired t test compared to control for each condition). d Quantitative RT-PCR expression analysis (by 2−∆∆Ct method) of control and infected bEnd5 cells shows upregulation of HIF-1 alpha and its transcriptional target gene VEGF upon a S. pneumoniae infection. Expression was normalized to ribosomal protein, large, P0 (RPLP0) that served as a housekeeping gene (N = 6, *p < 0.05, **p < 0.01, 2-tailed paired t test compared to control) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32529267), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) and Ponceau's Red stained membrane (b). Relative expression of aromatase in C and Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) and hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) and (h) 100 μm; and (e) and (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Subcellular localization of TM6SF2.A, primary hepatocytes from 8-week-old female mice on a chow diet were plated on collagen-coated coverslips for 4 h, fixed, and stained with antibodies against markers for the ER (calnexin (CANX)), cis-Golgi (receptor-binding cancer antigen expressed on SiSo cells (RCAS1)), and Golgi (Giantin (GOLGBI) (green, left column) and TM6SF2 (red, middle column)). The merged signal from both channels (yellow, right column) shows subcellular co-localization. All images were taken using a ×63 oil immersion objective. Scale bar, 10 μm. B, immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by immunoaffinity chromatography as described under “Experimental Procedures.” Microsome membranes were dissolved in RIPA buffer, and equal volumes were separated on 10% SDS-PAGE and immunoblotting as described under “Experimental Procedures.” BiP, binding immunoglobulin protein; Gos28, Golgi SNAP receptor complex member 1; *, nonspecific band. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27013658), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Gp, Pm-Or

Species Glossary

Reactivity: Hu, Mu, Gp, Pm-Or

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-192AF350
Alexa Fluor 405	NB200-192AF405
Alexa Fluor 488	NB200-192AF488
Alexa Fluor 532	NB200-192AF532
Alexa Fluor 594	NB200-192AF594
Alexa Fluor 647	NB200-192AF647
Alexa Fluor 700	NB200-192AF700
Alexa Fluor 750	NB200-192AF750
Allophycocyanin	NB200-192APC
Biotin	NB200-192B
DyLight 350	NB200-192UV
DyLight 405	NB200-192V
DyLight 488	NB200-192G
DyLight 550	NB200-192R
DyLight 594	NB200-192DL594
DyLight 650	NB200-192C
DyLight 680	NB200-192FR
DyLight 755	NB200-192IR
FITC	NB200-192F
HRP	NB200-192H
Janelia Fluor 549	NB200-192JF549
Janelia Fluor 646	NB200-192JF646
PE	NB200-192PE
PerCP	NB200-192PCP

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NB100-2600

BCL11B Antibody

(1 Review)

(7 Publications)

0.1 ml

NB100-2600

Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - Section of human skin tumor (higher magnification). Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:500. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.

Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - P7 brain mouse section was stained with anti-BCL11B antibody. Image from verified customer review.

Immunohistochemistry: BCL11B Antibody [NB100-2600] - Immunohistochemistry and FISH of AML cases. Immunohistochemistry analysis of BCL11B-expressing AML samples #59810, #57863 and #13 carrying t(2;14)(q22.3;q32.3), t(7;14)(q21q32) and no 14q32 alteration, respectively. BCL11B expression was detected in samples regardless of the presence of the 14q32 alterations. The expression was limited to the nucleus and the percentage of positive neoplastic cells was always >= 50%. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/12/1951), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - Section of human skin tumor. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:500. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.

Immunohistochemistry and FISH of AML cases. (B) Immunohistochemistry analysis of BCL11B-expressing AML samples #59810, #57863 and #13 carrying t(2;14)(q22.3;q32.3), t(7;14)(q21q32) and no 14q32 alteration, respectively. BCL11B expression was detected in samples regardless of the presence of the 14q32 alterations. The expression was limited to the nucleus and the percentage of positive neoplastic cells was always >= 50%. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31817495), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Pm, Gp, Pm, Pm-Or, Rb, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm, Gp, Pm, Pm-Or, Rb, Xp

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NB100-760

DDX19B Antibody

(4 Publications)

0.1 ml

NB100-760

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 binds directly to MKL1 RPEL domain. Recruitment of Ipobeta and Ddx19 from Ipobeta-depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (-), NIH 3T3 cytoplasmic lysate transfected with control siRNAs; (+), Ipobeta-depleted NIH 3T3 cytoplasmic lysate. Ponceau-stained full membrane (below). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Immunohistochemistry: DDX19B Antibody [NB100-760] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

<b>Biological Strategies Validation. </b>Western Blot: DDX19B Antibody [NB100-760] - Detection of Human and Mouse. Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 is specifically required for the nuclear localization of MKL1. Western blotting (WB) of cells treated with control (Ctrl) or Ddx19 siRNAs (si). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. Ddx19 recruitment from Hela cell lysate by GST-RPEL and GST-Ipobeta (left). WB, western blotting; (-), without Ran-Q69L; (+), with RanQ69L. Corresponding GST baits are stained with Ponceau to ensure the equal loading of the samples (below). Input sample corresponds to 5% of the Hela cell lysate used in the assay. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. GST-RPEL was used as a bait for pull down of Ddx19 and Ipobeta from the Hela cell lysate in the presence of increasing amounts of LatB-actin (0.25-10 uM) (left). Arrow indicates the increasing amounts of actin in the corresponding Ponceau staining (below). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Western Blot: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites. HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates and their expression was detected by HA-antibody. Co-precipitated endogenous Ddx19 was detected by western blotting (WB). Empty HA was used as a negative control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

MKL1 contains two Ddx19 interaction sites.(a) Co-IPs of endogenous MKL1 with endogenous Ddx19. NIH 3T3 cytoplasmic extract was immunoprecipitated with anti-Ddx19 antibody and the immunoprecipitates were blotted for indicated antibodies. Beads without antibody (no ab) were used as a negative control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

PFKFB3 promotes homologous recombination repair. a Confocal analysis of nuclear localization of PFKFB3 in U2OS cells following treatment with siRNAs (24 h) as indicated, subjected to IR (6 Gy, 2 h recovery), or left untreated, n = 3 independent experiments. Scale bar, 10 μm. To the right, bar chart showing PFKFB3 foci as quantified using CellProfiler, n > 100 cells/treatment. Data are displayed as means ± SEM. ***P 100 cells/condition. Data displayed as means ± SEM. ***P 500 cells/treatment. Data displayed as means ± SEM. ***P 5. ***P 100 cells/treatment. Data are displayed as means ± SEM. ***P 100 cells/condition. Data displayed as means ± SEM. ***P 500 cells/treatment. Data displayed as means ± SEM. ***P 5. ***P < 0.001; one-way ANOVA analysis Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30250201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

FKBP51 modulates EMT hallmarks. (a and b) FKBP51 silencing of SAN melanoma cells decreases TGF-beta and T beta RIII expression. Normalized expression rates (mean±S.D.) of TGF-beta (a, left) and T beta RIII (b, left) mRNA levels; (a, n=3; b, n=5). FKBP51 siRNA-treated sample expression=1. Western blot assay of TGF-beta in SAN melanoma cells treated with nonsilencing (NS) RNA or FKBP51 siRNA (a, right). Flow cytometric analysis of T beta RIII (endoglin) expression (b, right): FKBP51 siRNA-treated cells moved to the left relative to NS RNA-treated cells because of reduced staining (MFI: 111±10 and 64±3 in NS RNA and FKBP51 siRNA-treated cells, respectively). (c, and d) FKBP51 overexpression enhances TGF-beta and T beta RIII expression. Normalized expression of TGF-beta (c, left) and T beta RIII (d, left) mRNA in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1. (c, n=3; d, n=5). Western blot assay of TGF-beta levels in the same cells (c, right). Anti-Flag-labelled exogenous FKBP51. Flow cytometric analysis of T beta RIII expression (d, right). FKBP51-overexpressing cells moved to the right relative to WT or EV cells, indicating increased expression. (e) Graphic representation of cell counts ( × 10−3) in WT, EV-, or FKBP51-stably transfected melanoma cell cultures. Results are means of six independent experiments each performed in triplicate. (f) FKBP51 upregulates colony number and size. Colonies were counted under low magnification ( × 10) at four points on each dish. The data represent the means of two independent experiments performed in triplicate. Magnification: upper, × 10; lower × 40. (g) Boyden chamber filters invaded by melanoma cells. Filter was stained with Crystal Violet. OD value was obtained by reading the eluted Crystal Violet with a spectrophotometer at a wavelength of 570 nm. The increased OD in FKBP51 samples indicated that FKBP51-overexpressing cells invaded matrigel more efficiently than control cells. The data represent the means of three independent experiments performed in triplicate Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Gp, Pm-Or, Xp, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Gp, Pm-Or, Xp, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-27206

EMAP-II/AIMP1 Antibody

(2 Publications)

0.1 mg

NBP2-27206

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-27206] - Analysis of AIMP1 using AIMP1 antibody. Human HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse RAW cell lysate probed with 2 ug/ml of AIMP1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: EMAP-II/AIMP1 Antibody [NBP2-27206] - Staining in human pancreas tissue using AIMP1 antibody at 5 ug/ml.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, ChHa, Eq, Fe, Gp, Ma-Op, Pm, Am, Ch, Pm, Pm, Pm-Or, Rb, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, ChHa, Eq, Fe, Gp, Ma-Op, Pm, Am, Ch, Pm, Pm, Pm-Or, Rb, RM

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6801A

Alt Catalog# IMG-6801A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB100-61600

pre-mRNA cleavage factor I (59 kDa subunit) Antibody

(2 Publications)

100 ul

NB100-61600

Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - ICP4 containing complexes include TFIIH, cleavage and polyadenylation complex and chromatin remodeling complexes. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against CPSF2 and CPSF7 of the cleavage and polyadenylation complex were used. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.

Immunohistochemistry: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CPSF59 used at a dilution of 1:500 (2ug/ml). Detection: DAB

<b>Independent Antibodies Validation. </b>Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CPSF59 antibody NB100-61600 used for IP at 3 mcg/mg lysate. CPSF59 was also immunoprecipitated by rabbit anti-CPSF59 antibody NB100-61599, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CPSF59 used at a dilution of 1:250.

Deficient Cardiolipin Remodeling Causes Blunted HIF-1 alpha Response to Hypoxia(A) Total amounts of cardiolipin and monolysocardiolipin in wild-type (WT) and TAZKO MEF cells analyzed by mass spectrometry. Means ± SEMs, n = 4 per genotype. ∗∗p < 0.01.(B) Cell count of WT and TAZKO MEF cells cultivated in galactose-containing media. Means ± SEMs, n = 4, ∗p < 0.0001.(C) Analysis of gene expression of cytochrome c oxidase subunits (left) and canonical HIF-1 alpha target genes (right) in total mRNA isolated from WT and TAZKO MEF cells subjected to normoxia and hypoxia for 24 hr by qPCR. Means ± SEMs, n = 3, ∗p < 0.0294, ∗∗p < 0.0001. ns, non-significant.(D) Analysis of steady-state protein levels in whole-cell extracts of WT and TAZKO MEF cells after exposing to normoxia or hypoxia for the indicated time points by western blotting with antibodies against indicated proteins (left). Quantification of a signal ratio of HIF-1 alpha / beta -actin after 24 hr of hypoxia with WT normoxia set to 1 (right). Means ± SEMs, n = 3, ∗p < 0.04.(E) Analysis of HIF-1 alpha steady-state levels under hypoxia and normoxia in WT, TAZKO, and TAZKO Rescue MEF cells. Whole-cell lysates were analyzed by western blotting using antibodies against HIF-1 alpha and beta -tubulin.(F) HIF-1 alpha activity measurement using luciferase reporter assay, as described in the STAR Methods for indicated time points under normoxia or hypoxia. Means ± SEMs, n = 3, ∗p < 0.0001. ns, non-significant.(G) Steady-state protein levels analysis of whole-cell lysate from WT and TAZKO MEF cells after treatment with 2 mM DMOG or 50 μM CoCl2 for 24 hr by western blotting with antibodies against indicated proteins. n = 3.(H) Luciferase-reporter activity under control of an HIF-1 alpha promoter as described in the STAR Methods after administration of 2 mM DMOG or 50 μM CoCl2 for 24 hr. Means ± SEMs, n = 3, ∗p < 0.0005, ∗∗p < 0.005.(I) Western blot analysis of HIF-1 alpha steady-state levels in cells treated with 25 μM of proteasomal inhibitor MG132 under normoxia and hypoxia for 8 hr. Antibodies against GAPDH and ACTB were used as a control.(J) Determination of HIF-1 alpha degradation kinetics in WT and TAZKO MEF cells using cycloheximide chase for the indicated time points after 24 hr of hypoxia exposure. Quantitation of HIF-1 alpha protein levels compared to beta -tubulin in WT and TAZKO MEF cells. Means ± SEMs, n = 3.See also Figures S1 and S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30332638), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Ma, Pm, Mu, Rt, Ca, Pm, Eq, Gp, Pm-Or, RM

Species Glossary

Reactivity: Hu, Ma, Pm, Mu, Rt, Ca, Pm, Eq, Gp, Pm-Or, RM

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-61600AF350
Alexa Fluor 405	NB100-61600AF405
Alexa Fluor 488	NB100-61600AF488
Alexa Fluor 532	NB100-61600AF532
Alexa Fluor 594	NB100-61600AF594
Alexa Fluor 647	NB100-61600AF647
Alexa Fluor 700	NB100-61600AF700
Alexa Fluor 750	NB100-61600AF750
Allophycocyanin	NB100-61600APC
Biotin	NB100-61600B
DyLight 350	NB100-61600UV
DyLight 405	NB100-61600V
DyLight 488	NB100-61600G
DyLight 550	NB100-61600R
DyLight 594	NB100-61600DL594
DyLight 650	NB100-61600C
DyLight 680	NB100-61600FR
DyLight 755	NB100-61600IR
FITC	NB100-61600F
HRP	NB100-61600H
Janelia Fluor 549	NB100-61600JF549
Janelia Fluor 646	NB100-61600JF646
PE	NB100-61600PE
PerCP	NB100-61600PCP

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NBP2-27195

IMP Dehydrogenase 2/IMPDH2 Antibody

0.1 mg

NBP2-27195

Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody [NBP2-27195] - Analysis of IMPDH using IMPDH antibody. Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of IMPDH antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: IMP Dehydrogenase 2/IMPDH2 Antibody [NBP2-27195] - Analysis of IMPDH in human stomach tissue using IMPDH antibody at 5 ug/ml.

Reactivity:

Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, ChHa, Eq, Gp, Ma-Op, Pm, Rb, Pm, Fi, Pm-Or, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, ChHa, Eq, Gp, Ma-Op, Pm, Rb, Pm, Fi, Pm-Or, Ze

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6781A

Alt Catalog# IMG-6781A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NLS6731

CRLR Antibody - BSA Free

(1 Publication)

0.05 ml

NLS6731

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Vessel, endothelium

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Analysis of anti-CALCRL / CGRP Receptor antibody with colon, submucosal plexus and submucosal vessels at 34 ug/ ml.

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Analysis of anti-CALCRL / CGRP Receptor antibody with human bone, osteosarcoma.

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Anti-CALCRL / CGRP Receptor antibody IHC of human Lung, Non-Small Cell Carcinoma. Tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Breast, adenocarcinoma

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Brain Amygdala Neurons

Reactivity:

Hu, Rt, Po, Bv, Ca, Eq, Gt, Ha, Pm, Pm, Rb, Xp, Mu, Bt, Fi, Gp, Ma-Op, Pm-Or

Species Glossary

Reactivity: Hu, Rt, Po, Bv, Ca, Eq, Gt, Ha, Pm, Pm, Rb, Xp, Mu, Bt, Fi, Gp, Ma-Op, Pm-Or

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-27203

VprBP Antibody

0.1 mg

NBP2-27203

Western Blot: VprBP Antibody [NBP2-27203] - analysis of VPRBP using VPRBP antibody. Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of VPRBP antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: VprBP Antibody [NBP2-27203] - Intracellular analysis of VPRBP in human HeLa and mouse RAW cells using VPRBP antibody at 0.2 ug/10^6 cells. Red represents cells with VPRBP antibody, green represents isotype control antibody.

Reactivity:

Hu, Mu, Rt, Po, Ch, ChHa, Eq, Fe, Gp, Ma-Op, Pm, Rb, Bv, Pm-Or

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ch, ChHa, Eq, Fe, Gp, Ma-Op, Pm, Rb, Bv, Pm-Or

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6798A

Alt Catalog# IMG-6798A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-27204

LRH-1/NR5A2 Antibody

0.1 mg

NBP2-27204

Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: LRH-1/NR5A2 Antibody [NBP2-27204] - Simple Western lane view shows a specific band for LRH-1/NR5A2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Ca, Ch, Eq, Fe, Gp, Ma-Op, Pm, Am, Pm, Pm, Pm-Or, Rb, Sh

Species Glossary

Reactivity: Hu, Ca, Ch, Eq, Fe, Gp, Ma-Op, Pm, Am, Pm, Pm, Pm-Or, Rb, Sh

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6847A

Alt Catalog# IMG-6847A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-27080

EMAP-II/AIMP1 Antibody

0.1 mg

NBP2-27080

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-27080] - Analysis of AIMP1 using AIMP1 antibody. Human HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse Raw 4) 3T3 cell lysate probed with 2 ug/ml of AIMP1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: EMAP-II/AIMP1 Antibody [NBP2-27080] - Simple Western lane view shows a specific band for EMAP-II/AIMP1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Po, Bv, ChHa, Eq, Gp, Pm, Ch, Pm, Pm-Or, Rb, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, ChHa, Eq, Gp, Pm, Ch, Pm, Pm-Or, Rb, RM

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6802A

Alt Catalog# IMG-6802A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-27207

DDB1 Antibody

0.1 mg

NBP2-27207

Western Blot: DDB1 Antibody [NBP2-27207] - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: DDB1 Antibody [NBP2-27207] - Intracellular analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype control.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Pm, Pm, ChHa, Gp, Pm-Or, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Pm, Pm, ChHa, Gp, Pm-Or, RM

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6799A

Alt Catalog# IMG-6799A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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